A cell adhesion experiment using FluidFM
This application note shows an example of a single cell force spectroscopy experiment using FluidFM. It nicely demonstrates that FluidFM is capable of attaching a cell to a channeled cantilever in mere seconds by the application of underpressure via the channel, and of subsequently detaching the cell from the substrate surface it is adhered to and grows on.
In the FluidFM control software, a cell can be selected by placing a cross-hair over it. After this, the cantilever is automatically moved over the cell and spectroscopy starts.
Approach (blue) and retraction (red) curves of the adhesion experiment shown, as analyzed and graphed with SPIP.