AFM with spherical tips to determine the mechanical properties of cells, tissue, and substrates
Mechanical properties have shown to be important for fundamental cellular processes like migration and differentiation, as well as in the characterization and understanding of cancer. Although colloidal probes are commercially available, it is still common practice to glue beads on the cantilever. This is a precision job and can only be done one bead at a time. Coating has to be applied with the glued bead on the cantilever. Not so with the Flex-FPM system...
With FluidFM, gluing of beads is circumvented conveniently: pre-coated beads are mounted to the hollow cantilever shortly before the experiment by the application of underpressure. Upon contamination, a bead can be immediately exchanged and measurements can continue with the same cantilever.
The proof of principle of FluidFM colloidal spectroscopy has been demonstrated in Biophysical journal in 2013.
In 2016 an example has been published using colloidal particles prepared from polyanionic and polycationic recombinant spider silk proteins in the journal of physical chemistry C.
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- AFM force spectroscopy on a polymer blend
- Single molecule force spectroscopy of bacteriorhodopsin
- Spring constant calibration by frequency sweep
- Flex-ANA measurement on gelatin hydrogels
- Flex-ANA measurement on medical tubings
- Flex-ANA measurements on living cultured cells
- Single Cell Force Spectroscopy
- Bacterial adhesion force quantification by fluidic force microscopy
- Colloidal Properties of Recombinant Spider Silk Protein Particles
- Deformability Assessment of Waterborne Protozoa Using a Microfluidic-Enabled Force Microscopy Probe
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