Example of single-cell force spectroscopy using FluidFM

This application note shows an example of a single cell force spectroscopy experiment using FluidFM. It nicely demonstrates that FluidFM is capable of attaching a cell to a channeled cantilever in mere seconds by the application of underpressure via the channel, and of subsequently detaching the cell from the substrate surface it is adhered to and grows on.

single-cell force spectroscopy

In the FluidFM control software, a cell can be selected by placing a cross-hair over it. After this, the cantilever is automatically moved over the cell and spectroscopy starts.

Force-distance curves single-cell force spectroscopy

Approach (blue) and retraction (red) curves of the adhesion experiment shown, as analyzed and graphed with SPIP.

View application note (PDF)

Possible with

title strip=

Industrial solutions

In addition to our standard products we can design and develop custom-built atomic force microscope systems, stages, and parts. A dedicated team of highly qualified engineers is ready to create the optimal solution for your industrial application. For a first impression of what we can do, have a look at these examples.

title strip=

Flex-Axiom — AFM for materials research

By advancing key technologies and designs, Nanosurf has made the Flex-Axiom one of the most versatile and flexible atomic force microscopes ever, allowing a large variety of materials research applications to be handled with ease. Together with the powerful C3000 controller, complex material characterizations are possible.

title strip=

Flex-Bio — AFM for biology and life science

A key success factor in life science research is the combination of multiple techniques. With the Flex-Bio, Nanosurf's Bio AFM, you can combine the AFM imaging, spectroscopy and nanomanipulation capabilities of this system with the high-end optical imaging techniques available for inverted microscopes.