Example of single-cell force spectroscopy using FluidFM

This application note shows an example of a single cell force spectroscopy experiment using FluidFM. It nicely demonstrates that FluidFM is capable of attaching a cell to a channeled cantilever in mere seconds by the application of underpressure via the channel, and of subsequently detaching the cell from the substrate surface it is adhered to and grows on.

single-cell force spectroscopy

In the FluidFM control software, a cell can be selected by placing a cross-hair over it. After this, the cantilever is automatically moved over the cell and spectroscopy starts.

Force-distance curves single-cell force spectroscopy

Approach (blue) and retraction (red) curves of the adhesion experiment shown, as analyzed and graphed with SPIP.

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