Spotting and lithography with FluidFM

In cell biology, micropatterning is becoming a widely accepted technique to address cellular behavior at the single-cell level. The ability to guide cell growth by local suppression or stimulation, for example, is beneficial for studying cell growth, for developing cell-based sensors, and for tissue engineering applications. The production of microfabricated biosensors is another application that requires precise placement of biomaterials on a substrate.

FluidFM® technology by Cytosurge enables deposition of (bio)molecules and particles at defined locations with micrometer accuracy and with femtoliter volumes. The closed channel is capable of depositing molecules from a liquid in both air and liquid environment. This enables numerous applications in biomedicine, cell- and microbiology, as well as non-biological nanolithography.

Automated spotting calibration.

Automatically varying back pressure and contact time in a grid-like pattern (3 dots per condition), spot sizes can be quickly optimized.

Lithography using the FluidFM.

Nanosurf logo written in in air with a solution containing approximately 50% glycerol; back pressure 200 mbar.

View application note (PDF)