Flex-ANA — AFM for force mapping

The atomic force microscope that combines versatility and performance for life science

Techniques and applications for life science research
A design that opens up entirely new possibilities
Discover all the practical details that really matter in daily use
Discuss your application with one of our application scientists

A key success factor in life science research is the combination of multiple techniques. With the Flex-Bio, you can combine the imaging, spectroscopy and nanomanipulation capacity of this system with the high-end optical techniques available for inverted microscopes.

Techniques and applications for life science research

Flex-Bio comes with manual and motorized stages for seamless integration on Zeiss, Olympus, Nikon and Leica inverted microscopes or with standalone stages. On the inverted microscope, optical and AFM data can be correlated, as shown here for internal limiting membrane (ILM) of the human retina.

(A) Bright field image of isolated ILM in a physiological buffer. (B) Fluorescence image of the same section showing anti-laminin staining. (C) AFM topograph of a subsection of the ILM; also shown as overlay in B. (D) AFM stiffness map of the same subsection. The color for each point represents the local stiffness value as calculated from force curves recorded at the respective positions. (E) Histogram of the stiffness data shown in D. (F) Typical force-displacement curves obtained on the ILM and on the glass substrate. These curves are converted to force-indentation data, which then allows calculation of the stiffness. Stiffness distribution of biological tissues has been shown to be a marker for diseases such as age-related macular degeneration, arthritis and cancer. Data courtesy: Marko Loparic, Marija Plodinec, Philip Oertle, and Paul B. Henrich, Biozentrum/SNI/UHBS, University of Basel, CH.

For measurements that don’t need optical access from below, e.g. for the imaging and spectroscopy of samples like bacteriorhodopsin, a standalone stage makes the Flex-Bio compatible with the Nanosurf Isostage and Acoustic Enclosure 300, and generally makes the system much more compact.

A Flex-Bio system with stand-alone stage, isostage, and acoustic enclosure. (B) 2D crystals of bacteriorhodopsin [140 nm scan range]. (C) Power spectrum of B, showing a lateral resolution of well over 1 nm [dashed circle]. (D) Single molecule force spectroscopy of bacteriorhodopsin.

For more applications, please see the application and publication sections on the right, or visit our Applications page.

A system design that opens up entirely new possibilities

The Flex-Bio has a modular stage, cantilever holder, and software concept that allows an easy upgrade of the system to access many new possibilities in life science and materials research. Flex-FPM for cell and nano-manipulation, for example, and Flex-ANA for automatic nanomechanical analysis. In addition, advanced modes like MFM and KPFM that were originally developed for the Flex-Axiom system, are also available for Flex-Bio.

Flex-FPM
 
Flex-ANA
 
Flex-Axiom

Practical details that really matter in daily use

Cantilever holders with alignment structures are available for use with cantilevers containing alignment grooves. This provides micrometer repositioning accuracy, circumventing laser alignment and allowing you to find the same sample features again and again. Cantilevers enter the image from top to bottom, so sample orientation is always the same, no matter whether you look by eye, CCD camera, or AFM (when scanned at the default scan angle).

Alignment chip technology
 
Top view on stand-alone stage
 
Top view on inverted microscope, image courtesy: O. Guilaume-Gentil, ETH Zürich, Switzerland

Flex-Bio components and accessories

At the heart of the Flex-Bio system are the FlexAFM scan head and the C3000 controller with I100 interface box. On top of that, several options and accessories are available, designed to deliver stable and reliable results, or to provide access to new measurement possibilities and experiments for this BioAFM:

Inverted microscope stage (motorized or manual) with adapter plates for Zeiss, Olympus, Nikon and Leica
Cover slip holder with heating possibility
Environmental control boxes from several inverted microscope providers and Life Imaging Services (LIS)
Large acoustic enclosures and active vibration isolation solutions for the Flex-Bio/inverted microscope setup (e.g. from Accurion)
Standalone stages (motorized or manual) for measurements that don't require an inverted microscope setup
Acoustic enclosures to protect the standalone setup against acoustic disturbances and draft
Isostage for vibration-free measurements on the standalone setup
Flex-AFM video camera with simultaneous top and side view access

Free pre-sales consultancy from one of our experienced application scientists

Contact us now to discuss your application.

The Nanosurf advantage

Precision and performance
Flexibility through modularity
Practical design that matters

Latest Flex-Bio applications

Latest Flex-Bio publications

Flex-Bio brochures

Flex-Bio videos

Flex-Bio software

Innovation, Ease of Use, Reliability!

 

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