FlexAFM

Versatility and performance for biology and life science

For success in life science research, scientists depend on professional tools that can readily provide the information needed, regardless of the tasks at hand. By combining key technologies and components, Nanosurf has made the Flex-Bio system one of the most versatile and flexible AFM systems ever, allowing a large variety of biological and life science applications to be handled with ease. With the FlexAFM, you can combine the liquid AFM imaging, spectroscopy and nanomanipulation capacity of this system with the high-end optical techniques available for inverted microscopes.

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Flex-Bio application examples

Imaging of type I collagen fibrils

Collagen is the most abundant protein in mammals and contributes to more than 25% of the whole-body protein content. It is the main structural protein of the extracellular matrix of connective tissues and provides e.g. tendons and bone with their tensile strength. Most of the collagen found in mammals is fibrillar type I collagen. Type I collagen fibrils show a typical periodic morphology, the so-called D-banding. D-bands result from staggered self-assembly of individual collagen molecules into larger fibrils with a periodicity of about 67 nm.

Images of collagen fibrils from rat tendon were recorded in Prof. Snedeker's research group at the ETH Zürich. One of the reasearch areas of Prof. Snedeker is tendon mechanics and biology.

3D AFM topography of several type I collagen fibrils

AFM topography image of type I collagen fibrils

AFM deflection image recorded along with the topography image

The 3D representation of the AFM topography image nicely shows the typical periodic D-banding of type I collagen on all fibrils. The colloagen topography was recorded in static mode using a Nanosensors PPP-XYCONTR cantilever. AFM images were processed using Nanosurf Report Software. Preparation and imaging of collagen fibrils was performed by Massimo Bagnani, Prof. Snedeker research group, Uniklinik Balgrist, Institute for Biomechanics, ETH Zürich, Switzerland.

Measurements on living cultured cells

Mechanobiology is an emerging research area that deals with the effect of changing physical forces or changes in the mechanical properties of cells and tissues. Several diseases, such as fibrosis and atherosclerosis are associated with changes in tissue stiffness. Moreover, in cancer, the metastatic potential of cancer cells depends on their elastic modulus.

Here, the elastic modulus of living cells from a human breast basal epithelial cell line was measured using a Nanosurf Flex-Bio system with the Flex-ANA software.

Elastic modulus map

Unperturbed cell topography from force mapping

The first image shows the elastic modulus (in kPa) recorded on living breast epithelial cells immersed in cell culture medium. Differences in the elastic modulus within the cell can be clearly observed. The dark area surrounding the cells originate from the much stiffer cell culture dish substrate.

The second image shows the unperturbed cell topography extracted from the force mapping data. The topography is determined from the contact point of each force curve and thus shows the cell topography at zero applied force.

Elastic modulus mapped to the 3D topography

Elstic modulus distribution

Mapping the elastic modulus data to the 3D topography allows relating the information of both channels. The 3D image was generated using Gwyddion software.

The last image shows the distribution of the elastic moduli extracted from nanomechanical force mapping experiments. The peak at lower moduli corresponds to the stiffness of the cells. The peak at the right originates from the cell culture substrate and shows much higher elastic moduli.

AFM data courtesy of Philipp Oertle, Biozentrum, University Basel.

Single molecule force spectroscopy of bacteriorhodopsin

The force-distance curve below reports the controlled C-terminal unfolding of a single bacteriorhodopsin (BR) membrane protein from its native environment, the purple membrane from Halobacterium salinarium.

Solid and dashed orange lines represent the WLC curves corresponding to the major and minor unfolding peaks observed upon unfolding BR, respectively. The contour length of the stretched polypeptides of the major unfolding peaks is given in amino acids (aa).

Single molecule force spectroscopy of bacteriorhodopsin

This data was recorded using a (10-µm; version 3) in combination with the C3000 controller and a cantilever with a nominal spring constant of 0.1 N/m (Uniqprobe, qp-CONT, Nanosensors).

The most flexible atomic force microscope for materials research

For success in materials research studies, scientists depend on professional tools that can readily provide the information needed, regardless of the tasks at hand. By advancing key technologies and designs, Nanosurf has made the Flex-Axiom one of the most versatile and flexible AFMs ever, allowing a large variety of materials research applications to be handled with ease. In combination with the powerful C3000i controller, complex material characterizations are possible.

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Topography of SrTiO₃ in dynamic mode

Strontium titanate (SrTiO₃, STO) is an oxide of titanium and strontium exhibiting a perovskite structure. It has interesting and partly unique material properties. It is used as substrate for growth of oxide-based thin films and high-temperature superconductors.

STO forms surfaces that show a layered structure. The thickness of individual layers is in the range of a few Angstrom. Atomic force microscopy is an ideal tool to image and measure these structures.

Topography showing steps of strontium titanate; image size 1.1µm

Section profile and height distribution

The sample clearly shows the typical layer structure STO. Here, the layers are not perfectly smooth, but exhibit residual roughness of approx. 125 pm (RMS). This is caused by a non-ideal termination process during the preparation of this STO sample.

The graph shows the profile of the image shown above along a line extending from the top left to the bottom right corner of the imaged area. The profile also clearly shows the layered structure of the sample and reveals step heights of approx. 4 Å. Similarly in the right panel, the height distribution histogram of the image above clearly shows approx. 4 Å-spaced peaks for the different layers of the sample.

Topography and KPFM of CVD grown molybdenum disulfide monolayers

In this application note, monolayer MoS₂ grown by chemical vapor deposition (CVD) was imaged with Kelvin probe force microscopy (KPFM) using a Flex-Axiom to study the contact potential difference variation on a single crystal.

Monolayers of MoS₂ were grown on a silicon substrate by chemical vapor deposition (Sample courtesy: University of Illinois – Urbana-Champlain).

Non-uniformity of the contact potential signal across the monolayer can inform about doping profiles and other surface defects.

MoS₂ monolayers optical micrograph

a) AFM topography image of single MoS₂ monolayer. Location where profile is taken indicted by red line.
b) Height (top) and KPFM voltage (bottom) profile across monolayer

Measurements using the Flex-Axiom show a step height of 0.6 nm for the MoS₂ monolayer. Concurrent KPFM measurements show a 650 mV contact potential difference between the monolayer and the SiO₂ substrate.

3D AFM topography overlay MoS₂

All measurements were performed using a Flex-Axiom system equipped with a ANSCM-PA cantilever from AppNano. Images were processed using MountainsMap SPM.

For more information contact our application scientists

KPFM and MFM on stainless steel

In the experiment shown below, KPFM and topography data were recorded in a single run using a Flex-Axiom system. Also see the related .

KPFM overlay on a topography image of stainless steel
Scan size: 80 µm x 80 µm
Potential range: 200 mV

The topography image itself
Scan size: 80 µm x 80 µm
Height range: 50 nm

MFM image of the same area
Scan size: 80 µm x 80 µm
Phase range: 10°

The established microfluidic tool for nanomanipulation and single-cell biology

FluidFM probe microscopy (FPM) combines the force sensitivity and positional accuracy of an AFM with FluidFM technology by Cytosurge to allow a whole range of exciting applications in single-cell biology and nanoscience.

Nanosurf has the longest experience providing AFM systems with the FluidFM^® add-on, as Cytosurge's initial cooperation partner for this innovative technology - the FlexAFM with FluidFM^® system was launched in 2013. FluidFM add-ons are available for the FlexAFM and CoreAFM platforms and a unique integrated FPM solution on the FlexAFM.

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 Highly accurate pressure, force, and position control with optical sample access

• Fully integrated system with user-friendly FluidFM^® ARYA operator software
• FluidFM® microfluidics control system
• Compatible with major inverted microscope brands

 Different FluidFM^® probes: hollow cantilevers designed for specific applications

• FluidFM® micropipettes: tipless cantilevers with opening at the cantilever end
• FluidFM® nanopipettes: cantilevers with opening at the tip apex
• FluidFM® rapid prototyping probes: cantilevers with closed pyramidal tips, ready for FIB milling

 Pioneering research within reach

• A tool to conduct original research at the frontiers of science

FluidFM opens the door to fascinating new experiments

single cell adhesion

colloidal spectroscopy

single cell injection

spotting

single bacteria adhesion

single cell isolation

single cell extraction

nanolithography

An integrated solution with optical, force, and fluidic control

Optical cell selection by fluorescence properties (left image). The cantilever was gently placed on the cell under force control and trypsin administration was monitored from a co-deposited fluorescence dye (blue). After release, the cell was picked up with the same cantilever and isolated from the rest by placing it in a separate well, sorted by fluorescence (right image; adapted from Lab Chip (2014) 14, 402‑414 with permission from The Royal Society of Chemistry). Data courtesy: O. Guilaume-Gentil, ETH Zürich, Switzerland.

Innovative and intuitive handling

With its touchscreen interface and predefined experimental workflows, e.g for single-cell injection and extraction or for bacterial and cell adhesion force measurements, the intuitive FluidFM ARYA operator software will guide you through each of your experiments step-by-step.

All FluidFM probes come in sterile blister packs and pre-mounted on plastic carrier clips. Each blister pack has a QR code that can be read with the QR code reader supplied with the Flex-FPM system to allow direct import of detailed cantilever information into the operator software.

To learn more details, please visit the FluidFM page.

ANA: The perfect tool for fully automated nanomechanical data acquisition and analysis

The ANA system is an automated solution for AFM-based nanomechanical analysis. It is designed to investigate the nanomechanical properties of materials such as cells, tissues, scaffolds, hydrogels, and polymers on multiple or large samples via force spectroscopy and force mapping in an intuitive and automated fashion.

ANA is compatible with Nanosurf's FlexAFM scan heads: for materials science, as well as for life science applications on a setup on an inverted microscope.

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Nanomechanical analysis of large or multiple samples

The combination of a straight-forward workflow-based experimental procedure in the ANA software, carefully selected hardware elements and motorization, and well-designed and thought-through control algorithms are hallmarks of the Flex-ANA system. Measurement locations on large or multiple samples are defined by simply clicking on an overview image with a large field-of-view that covers the entire accessible 32 mm × 32 mm sample area. Height differences of up to 5 mm can be easily overcome by the system, and many types of nanomechanical measurements, analyses and models can be performed and used!

Handle demanding samples with ease

During an experiment, the ANA system coordinates the movement of the scan head and the motorized XYZ translation stage (range: 32 mm × 32 mm × 5 mm) to cope with large sample height variations. The system can optionally be equipped with an additional 100-µm Z piezo for an extended force spectroscopy range. Thus, samples with macroscopic and microscopic roughness in the range of several millimeters and micrometers, respectively, as well as soft and sticky samples, can be easily addressed.

Learn about details in our webinar by Dr. Edward Nelson

Learn about the automated nanomechancial analysis module and its application in analyzing blended polymers. See the ease of use in data collection and analysis, and how automated collection of data from multiple samples has the potential to save time and make your research more efficient.

Operation by experts as well as novice users

The ANA control software provides a Basic and an Expert user mode that are designed for different degrees of user experience and training:

In Basic mode, less experienced users are guided through the setup and calibration of the ANA system step-by-step. Optimized workflows guarantee that everything is done in the correct sequence, that nothing is forgotten, and that all is set properly.

In Expert mode, experienced users have more freedom in operating and setting up the system to suit their individual needs. Measurement parameters can also be prepared and stored by an expert, and then subsequently used by less experienced users in Basic mode.

The ANA software was partly developed within the framework of the OYSTER project, which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 760827.

To learn more details, please visit the ANA page.

FlexAFM modes, specifications and dimensions

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Some modes may require additional components or software options. For details, please view the brochure or contact us.

System specifications

Scan head dimensions