Research AFM with a competitive price tag Integrated system with small footprint 33 modes and functions Easy handling
The CoreAFM is the result of intelligently combining the core components of AFM to achieve maximum versatility and user-friendliness. Due to this fundamental design approach, the CoreAFM is equipped to perform AFM at its best.
The fusion of a modern flexure-guided scanner, XYZ sample stage, camera, active vibration isolation table, and airflow shielding in a single all-in-one unit results in a complete AFM system with an unparalleled compact footprint. The system comes with a fully digital 24-bit controller developed specifically for the CoreAFM scanhead. All the essential functions of modern AFM are integral components of the CoreAFM system; all you need to do to take the CoreAFM into operation is connect the controller, and plug in power and USB.
Seamlessly extendable functionality
Thirty-two standard and optional modes with fully compatible add-ons make the CoreAFM the tool of choice for applications ranging from materials research to life science and electrochemistry. Starting from the basic CoreAFM system, its functionality can be seamlessly extended. Advanced AFM modes and functionality like sample heating, environmental control, scripting, and many others can easily be added to your CoreAFM. For details and dependencies, see the overview graphic.
Easy setup and handling
See for yourself how easy it is to prepare and operate the CoreAFM. Watch the video for a demonstration of the main features, and a brief tutorial on how to perform a measurement using this system.
Spike-Guard
Deeper system integration of the Isostage is reflected in the unique Spike-Guard, which eliminates glitches during imaging. Although the Isostage is an active vibration isolation system, glitches can still occur when distortions are too severe. Spike-Guard detects such anomalies and rescans the line for a distortion-free image.
Powerful and versatile
State of the art electronics with 24-bit ADC and DAC ensure high-resolution XYZ driving of the 100×100×12 µm scanner and allow for low-noise force detection limited only by the cantilever. Thirty-two standard and optional modes with fully compatible add-ons make the CoreAFM the tool of choice for applications ranging from materials research to life science and electrochemistry. Starting from the basic CoreAFM system, its functionality can be seamlessly extended.
A closed scanner compartment features acoustic and air current isolation, while sample stage positioners still allow you to adjust your sample
When the scanner compartment is opened, it provides access to the scanner and sample stage, e.g. to place a new sample for measurement
AFM Modes
CoreAFM imaging modes
This overview shows which modes the instrument is capable of. Some modes may require additional components or software options. For details, please view the brochure or contact us directly.
Standard imaging modes
Static Force Mode
Lateral Force Mode
Dynamic Force Mode (Tapping Mode)
Phase Imaging Mode
Magnetic properties
Magnetic Force Microscopy
Electrical properties
Conductive AFM (C-AFM)
Piezoelectric Force Microscopy (PFM)
Electrostatic Force Microscopy (EFM)
Kelvin Probe Force Microscopy (KPFM)
Mechanical properties
Force Spectroscopy
Force Modulation
Stiffness and Modulus
Adhesion
Unfolding and Stretching
Force Mapping
Lithography and Nanomanipulation
Electrochemical AFM (EC-AFM)
Applications
CoreAFM application examples
Kelvin probe force microscopy (KPFM) on graphene
Graphene belongs to the category of 2D materials and is of interest in the research and development of new devices and materials. Besides atomic resolution imaging of graphene to learn more about crystal orientation, edges and defects, the functional properties of graphene are also of great interest.
In this application note, multilayer graphene was imaged with Kelvin probe force microscopy (KPFM) using a CoreAFM to study the contact potential difference variation on a single flake.
Optical image recorded on an upright microscope with a 50x NA0.9 objective. Inset: area where KPFM data and AFM images of graphene were recorded
Topview image of same area in CoreAFM
Multilayer graphene flakes were generated by mechanical exfoliation of graphite and subsequent transfer to a silicon-silicondioxide substrate. The KPFM measurement was carried out in single-run mode, recording the contact potential during the scanning of the topography.
Thin flakes were localized on the substrate with an upright microscope. Using markers on the substrate, the same flakes were placed under the cantilever using the topview camera of the CoreAFM, after which AFM images of graphene and KPFM data were recorded.
Overlay of contact potential difference on an AFM topography image of a multilayer graphene flake.
Sample courtesy: Hiske Overweg, Klaus Ensslin, ETH Zürich, Switzerland
All measurements were performed using a CoreAFM system equipped with a PPP-EFMR cantilever from Nanosensors. AFM images of graphene were processed using the MountainsMap SPM.
Out of plane piezoresponse force microscopy (PFM) on Lithium Niobate
Lithium Niobite (LiNbO3) is an optically transparent, piezo responsive material used, for example, in piezo sensors or in mobile phones.
We tested a LiNbO3 sample (PFM03, obtained from TipsNano, Estonia) with CoreAFM. The sample has a regular domain structure with 10-µm period. The spontaneous polarization has the opposite direction in the neighboring domains.
To measure the piezoresponse during imaging, a 7.5 V AC voltage was applied to the cantilever while raster scanning the tip over the sample. In response to the applied voltage, the sample periodically expands and shrinks, either in phase with or in counter phase to the excitation frequency. Despite a small measured RMS roughness of 0.3 nm, the domains could not be recognized in the topography. The domains could however be readily identified in the out of plane piezoresponse.
Lithium Niobite (LiNbO3) is an optically transparent, piezo responsive material used, for example, in piezo sensors or in mobile phones. We tested a LiNbO3 sample (PFM03, obtained from TipsNano, Estonia) with CoreAFM. The sample has a regular domain structure with 10-µm period. The spontaneous polarization has the opposite direction in the neighboring domains.
PFM on Lithium Niobate
To measure the piezoresponse during imaging, a 7.5 V AC voltage was applied to the cantilever while raster scanning the tip over the sample. In response to the applied voltage, the sample periodically expands and shrinks, either in phase with or in counter phase to the excitation frequency. Despite a small measured RMS roughness of 0.3 nm, the domains could not be recognized in the topography. The domains could however be readily identified in the out of plane piezoresponse.
Options & Accessories
CoreAFM options and accessories
Functional sample holders give you broad functionality
The CoreAFM and its accessories have been designed as part of a consistent system concept.
Top center: environmental option
Top row: standard sample holder, C-AFM sample holder, heating sample holder, heating/cooling sample holder
Bottom row: variable magnetic field generator, EC-AFM sample holder, Petri-dish holder, 150 µm Z-actuator sample holder
The result is an integrated, high-quality, high-performance, and versatile AFM system that offers the best price-to-performance ratio on the market. An atomic force microscope that is easy to use, robust, and easily extendable.
By configuring the CoreAFM with accessories and options for your applications, you can focus on the essence of AFM for your research: the measurements your application requires, not finding ways of making your instrument compatible.
Integrated inverted microscope functionality
View samples from below
Exchangeable objective lens
Enhances FluidFM® functionality
The CoreAFM is a very versatile atomic force microscope that can be used for a multitude of different types of research. The digital inverted microscope option (DIMO) enhances your CoreAFM to allow optical view from below, replacing the need for a completely different AFM setup with an inverted
microscope.
Enhanced viewing ability
The DIMO empowers you to conduct advanced experiments that require an inverted microscope, as is the case for many Bio AFM applications. It enhances your capabilities when using accessories like the petri dish holder (visible at the beginning of the video to the right), and does not obstruct any standard accessories that fit your CoreAFM (with the exception of the variable magnetic field sample holder).
Fluorescence measurements
The DIMO uses the internal illumination source for transmission optical microscopy. With the fluorescence option, light from an optical fiber can be easily guided into the CoreAFM for epi-illumination from the bottom.
AFM data can be overlaid with the optical data to colocalize information from each imaging mode. The image shows an overlay of AFM topography with fluorescence (green) and transmission light (gray) optical microscopy images. The overlay was created with MountainsMap image processing software.
Even better with FluidFM® functionality
Nanosurf has a long-standing collaboration with Cytosurge and has been developing FluidFM® solutions for FlexAFM since 2011, offering powerful applications for single-cell analysis and manipulation.
The DIMO upgrades the CoreAFM to be capable of performing FluidFM® on biological samples too.
Below is an example of FluidFM® colloidal spectroscopy on HeLa cells: the powerful digital microscope allows you to easily view cells, maneuver the cantilever, and carry out your single-cell manipulations.
(Left) Optical image of cantilever with attached colloid taken with DIMO. (Right) Elastic modulus of HeLa cell measured with FluidFM® colloidal spectroscopy
Motorized function for cantilever centering and focus
FluidFM® spotting observed by DIMO
Single cell injection with FluidFM
High-powered microscope optics
Objective: 20× 0.4 NA long working distance objective from Nikon
Objective is interchangeable between experiments with other Nikon objectives that have a M25×0.75 thread and 60-mm parfocal length
Camera: Highly sensitive 2.3 MP monochrome CMOS camera with 5.86 µm × 5.86 μm pixel size (eq. 1/1.2”), USB 3.0, frame rate up
to 48 frames/s at full size
Fluorescence option with fiber coupling and filter set that can be changed between experiments
Filters are in a Nikon filter block, as used for Eclipse Ti series microscopes
Optical resolution: 0.8 µm with default objective and transmission
Motorized focusing and cantilever centering
0.8 μm resolution with 20× 0.4 NA objective shown on USAF 1951 Resolving Power Test Target
The established microfluidic tool for nanomanipulation and single-cell biology
FluidFM probe microscopy (FPM) combines the force sensitivity and positional accuracy of an AFM with FluidFM technology by Cytosurge to allow a whole range of exciting applications in single-cell biology and nanoscience.
Nanosurf has the longest experience providing AFM systems with the FluidFM® add-on, as Cytosurge's initial cooperation partner for this innovative technology - the FlexAFM with FluidFM® system was launched in 2013. FluidFM add-ons are available for the FlexAFM and CoreAFM platforms and a unique integrated FPM solution on the FlexAFM.
Highly accurate pressure, force, and position control with optical sample access
Fully integrated system with user-friendly FluidFM® ARYA operator software
FluidFM® microfluidics control system
Compatible with major inverted microscope brands
Different FluidFM® probes: hollow cantilevers designed for specific applications
FluidFM® micropipettes: tipless cantilevers with opening at the cantilever end
FluidFM® nanopipettes: cantilevers with opening at the tip apex
FluidFM® rapid prototyping probes: cantilevers with closed pyramidal tips, ready for FIB milling
Pioneering research within reach
A tool to conduct original research at the frontiers of science
FluidFM opens the door to fascinating new experiments
An integrated solution with optical, force, and fluidic control
Optical cell selection by fluorescence properties (left image). The cantilever was gently placed on the cell under force control and trypsin administration was monitored from a co-deposited fluorescence dye (blue). After release, the cell was picked up with the same cantilever and isolated from the rest by placing it in a separate well, sorted by fluorescence (right image; adapted from Lab Chip (2014) 14, 402‑414 with permission from The Royal Society of Chemistry). Data courtesy: O. Guilaume-Gentil, ETH Zürich, Switzerland.
Innovative and intuitive handling
With its touchscreen interface and predefined experimental workflows, e.g for single-cell injection and extraction or for bacterial and cell adhesion force measurements, the intuitive FluidFM ARYA operator software will guide you through each of your experiments step-by-step.
All FluidFM probes come in sterile blister packs and pre-mounted on plastic carrier clips. Each blister pack has a QR code that can be read with the QR code reader supplied with the Flex-FPM system to allow direct import of detailed cantilever information into the operator software.
To learn more details, please visit the FluidFM page.
Required by default
Special cantilever holders without alignment grooves are available
Resonance frequency dynamic mode
< 4 MHz
Cantilever shape
Single rectangular cantilevers and multilever cantilevers (depending on scan head version and cantilever holder)
Chip thickness
300 μm, 500 μm or 600 μm depending on cantilever holder
System dimensions
Software
Control software
The control software for Nanosurf AFMs is an intuitive platform made for performing your AFM measurements efficiently and easily. Our Service team and software engineers are constantly developing and implementing new features and enhancements to further improve the user experience. We regularly publish new and improved versions, which you can download for free. You can install the software on as many computers as you wish to analyze your data.
Free lifetime updates: download all software updates for free
All software updates for Nanosurf control software are free of charge. Our software team is constantly working on new features and improvements to make the user experience better, more intuitive and more efficient.
Software features
Automatic/parameter-free frequency tuning based on cantilever characteristics
Simply choose the cantilever you are using, and the system automatically performs the frequency sweep prior to approaching the sample. No manual setting of parameters is required.
Distance measuring tools: measure the distance between points or lines, the height of features, and more
A selection of different measuring tools allow you to accurately measure angles and distances directly on the acquired measurement image.
Determine the distance between two points or between two parallel lines to make very precise measurement (as shown in the video).
More convenient usability features:
Spectroscopy wizard: follow easy steps to set up spectroscopy measurements
One software UI for all scan heads: no additional learning curve if you use multiple Nanosurf AFM systems
Automated deflection calibration
UI layouts for beginners and advanced users
Highly configurable graph area with mode-dependent auto-layout: the software automatically shows the relevant graphs and information
Easy file handling with comfort features: auto apply naming conventions, Windows Explorer integration, image gallery, bulk renaming.
Includes a powerful scripting interface: automate and extend capabilities according to your needs. Compatible with most programming environments (e.g. LabView, Python, MatLab, C++, Java, and more)
