How to measure mechanics of single cells

06.11.2024
The ultimate tool for nanoscale research from biological molecules to advanced new materials.
The versatile mid-range research AFM that grows with your demands in modes and accessories.
A compact affordable research AFM that is astoundingly easy to use, with more than 30 modes and options.
Measure roughness and other material properties of heavy and large samples up to 300 mm and 45 kg.
Bringing the power of DriveAFM to a wafer metrology system purpose-built for the requirements of the semiconductor industry.
For unique requirements, we will design a bespoke AFM solution, leveraging our decades of engineering expertise.
Slide an AFM onto your upright optical microscope turret for a leap in resolution.
One of the smallest ever AFMs, created for integration into custom stages or existing setups.
A flexibly mountable research-grade scan head for integration into custom stages or existing set ups.
What is atomic force microscopy (AFM)? How does AFM work? What AFM modes do I really need? How do I get started with AFM?
Learn how AFM works with cantilever/tip assembly interacting with the sample. Explore CleanDrive technology, calibration methods, and feedback principles for precise nanoscale imaging.
An overview of common AFM modes. To learn about each mode in more detail and see application, view the full article.
We regularly publish detailed reviews providing practical guidance and theoretical background on various AFM applications.
Read detailed technical descriptions about selected AFM techniques and learn how to perform specific measurements on Nanosurf instruments.
A library of links to research papers in which Nanosurf instruments were used.
Learn AFM from our library of recorded webinars, covering different measurement techniques, modes, and areas of application.
Short video clips explaining how to perform different operations on Nanosurf instruments.
Watch a product demonstration to learn about the capabilities of our AFMs.
Short videos of our AFMs.
Browse news articles, press releases and a variety of other articles all around Nanosurf.
Browse Héctor Corte-Léon's weekly experiments, for inspiration, entertainment, and to discover everyday applications of AFM.
By guest author Alessandro Pitani, Paul-Drude-Institut für Festkörperelektronik, Leibniz-Institut im Forschungsverbund Berlin e. V.
A. Pitanti1,2,3*, M. Yuan1, S. Zanotto3 and P. V. Santos1*
1Paul-Drude-Institut für Festkörperelektronik, Leibniz-Institut im Forschungsverbund Berlin e. V., 5-7, Hausvogteiplatz, Berlin, 10117, Germany
2Dipartimento di Fisica “E. Fermi”, Universit`a di Pisa, Largo B. Pontecorvo 3, I-56127 Pisa, Italy
3NEST, CNR Istituto Nanoscienze and Scuola Normale Superiore, piazza San Silvestro 12, 56127, Pisa, Italy
*Email: alessandro.pitanti@unipi.it, santos@pdi-berlin.de
Link to publication: High-resolution acoustic field mapping of gigahertz phononic crystals with atomic force microscopy.
#Done with a FLEX: The most flexible atomic force microscope for materials research
The last decades have witnessed wide research activities focused on the integration of acoustic technologies in photonic and electronic devices for improving existing functionalities and enabling novel ones. Surface acoustic waves (SAWs) have played a prominent role in this trend: their high frequency, along with their large quality factors in simple delay line configurations, make them very efficient spectral filters and oscillators in telecommunication applications, manifested by their diffuse use in mobile devices.
A more complex manipulation of SAW will show even further achievements and could be leading the transition to the sixth generation of telecommunication technologies: one can imagine, along with an increase in operating frequency, an enhancement of the SAW-embedded data transmission rate through wave manipulation with complex spatial or frequency multiplexing thus allowing simultaneous on-chip operations, such as filtering, on several communication channels. These complex functionalities are already studied in laboratory environment in proof-of-concept experiments, showing exciting results such as acoustic holography [1], negative acoustic refraction [2], as well as several topological effects [3], igniting the effort towards their validation in commercial platforms.
With increasing device frequency and design complexity, the need arises for faster and versatile tools for characterizing acoustic fields at tens of nanometer resolution, improving the existing experimental benches including electrical and optical probes. To this end, we recently demonstrated the implementation of a characterization technique based on atomic force microscopy (AFM) to study the complex wave manipulation properties of phononic crystals (PhC); this is done by measuring the acoustic fields excited by SAWs simultaneously with high-resolution AFM topography (see Ref. [4] for details).
The basic concept behind the so-called Acoustic Atomic Force Microscopy (AAFM) lies in the non-linear interaction between an AFM tip and the sample surface. Given an out-of-plane surface modulation induced by a mechanical wave with amplitude and angular frequency, it is possible to demonstrate that the cantilever deflection “slow” response (as averaged for a time ) carries a contribution coming from the “fast” mechanical wave, despite being the SAW frequency much larger than the inverse of the averaging time :[5]
<d>T=1/κF(z)+1/κ(F''(z)F'(z)/(F'(z)+κ)+F''(z)/2)A2 (1)
Here, is the spring constant and the force-distance curve, and its first and second derivatives, respectively. The second right-hand term in Eq. (1) gives a direct evaluation of the mechanical wave intensity , which can be better extracted by demodulating the mechanical signal under an appropriate amplitude modulation [4] (see Fig. 1 a).
This last step of AAFM technique can be straightforwardly implemented in the Nanosurf FlexAFM, which already includes a secondary lock-in with versatile input-output, in our case used for evaluation of acoustic wave intensity within the very same scan registering sample topography.
Exploiting the fast scanning capabilities of AFM along with the tens of nanometer and below resolution, we characterized GaAs membrane devices with SAW excitation induced via the piezoelectric effect with interdigitated transducers (IDT). A scanning electron microscopy of one of the investigated devices is reported in Fig. 1 (b-c).
We investigated SAW vibrations around 1 GHz in standing wave configuration, assessing their scattering with non-homogeneous interfaces and defects; moreover, we investigated the role of unit cell symmetry for the Bloch modes of PhC in the same frequency range, showing how the patterning induce a strong modification of the density of states resulting in possible, complex manipulation of the mechanical waves.
A typical measurement of a PhC is shown in Figure 1 (d-e). While the topography shows few unit cells comprising rectangular holes in a square lattice on a GaAs membrane, the extracted vibrations exhibit a complex pattern, given by the superposition of multiple, scattering-induced Bloch modes, which can be investigated at a single unit cell level, already overcoming the diffraction-limited lateral resolution of interferometric techniques. More details can be found in [4].
References
[1] M. Xu, W. S. Harley, Z. Ma, P. V. S. Lee, and D. J. Collins, Sound-speed modifying acoustic metasurfaces for acoustic holography, Advanced Materials 35, 2208002 (2023) https://doi.org/10.1002/adma.202208002
[2] S. Zanotto, G. Biasiol, P. Santos, and A. Pitanti, Metamaterial-enabled asymmetric negative refraction of Ghz mechanical waves, Nature Communications 13, 5939 (2019) https://doi.org/10.1038/s41467-022-33652-8
[3] J. Cha, K. Kim, and C. Daraio, Experimental realization of on-chip topological nanoelectromechanical metamaterials, Nature 564, 229 (2018) https://doi.org/10.1038/s41586-018-0764-0
[4] A., M. Yuan, S. Zanotto and P. V. Santos, High-resolution acoustic field mapping of gigahertz phononic crystals with atomic force microscopy, Phys. Rev. Applied 20, 054054 (2023). https://doi.org/10.1103/PhysRevApplied.20.054054
[5] J. Hellemann, F. Müller, M. Msall, P. V. Santos, and S. Ludwig, Determining amplitudes of standing surface acoustic waves via atomic force microscopy, Phys. Rev. Appl. 17, 044024 (2022) https://doi.org/10.1103/PhysRevApplied.17.044024
06.11.2024
How to measure mechanics of single cells
14.05.2024
How to measure forces between droplets
11.03.2024
How to perform mass measurements in cell exposed to different environments.
18.04.2024
FridayAFM: See how one of our Industrial AFMs can be used to look at the surface of mobile phones.
28.03.2024
Explore the fascinating world of AFM experiments with bismuth oxide layers in this detailed blog post. Learn about ...
21.03.2024
FridayAFM: Point contact diode on lead sulfide.
Interested in learning more? If you have any questions, please reach out to us, and speak to an AFM expert.