Discover the capabilities of WaveMode AFM in characterizing bottlebrush polymers with unprecedented detail and speed, ...
Polymers are essential components in many devices and materials used in everyday life, often without us noticing. Materials such as polyethylene (PE), polypropylene (PP), and polyethylene terephthalate
(PET) are commonly found in food containers, wrapping films, and bottles. Polymers also play a key role in the organic LED screens of smartphones, while synthetic fibers like polyester and nylon are widely used in clothing. In industrial applications, polymers are valued for their surface properties, including permeability control, mechanical durability, and light reflectivity.
Besides all these popular applications, bottlebrush polymers are rapidly gaining attention in the field of nanomaterials science. With their densely grafted side chains radiating from a central backbone, these unique macromolecules resemble molecular bristles - a structure that gives them remarkable properties. Their elongated cylindrical shape and lack of entanglement make them ideal for applications ranging from supersoft elastomers and photonic materials to biomedical devices, energy storage, and even films for lithography masks. What makes bottlebrush polymers especially exciting is their tunability: by adjusting the chemical makeup of the backbone and side chains, researchers can fine-tune viscoelastic behavior, thermal processability, and alignment characteristics. Cutting-edge synthetic techniques now allow scientists to design these polymers with precise control over size, composition, and architecture. Still, challenges remain, particularly in monitoring the number and shape of the side chains, which determine the material properties.
A bottlebrush polymer deposited on mica in liquid environment imaged with DriveAFM in WaveMode.
It is possible to resolve the backbone and the individual side chains.
The properties of bottlebrush polymers are closely linked to their molecular conformation. To fully understand these materials, researchers need a technique capable of imaging individual polymer molecules directly on the surface of interest, with the highest resolution in all three spatial dimensions. Atomic Force Microscopy (AFM) is well-suited for this purpose. However, not every instrument on the market can capture high-resolution images of bottlebrush polymers. With side chains spaced only few nm apart, every detail matters, including speed. The sample must reach equilibrium with the AFM tip and the rest of the system, but these conditions are disturbed by the instrument itself. Slow scanning speeds can lead to significant drift, distorting the image and making it difficult to resolve molecular features. Even under ideal conditions, perfect equilibration is nearly impossible, and drift remains a persistent challenge at the nanometer scale.
To address this limitation, Nanosurf has developed WaveMode off-resonance imaging for its high-end DriveAFM system. Being up to 15 times faster than similar modes in ambient conditions, WaveMode overcomes the effects of drift through shorter imaging times.
WaveMode is the fastest off-resonance AFM mode available, enabled by the photothermal effect. This effect transfers energy from an infrared laser beam to the cantilever, allowing off-resonance oscillation without the limitations imposed by the f/Q ratio in dynamic mode.
For example, when bottlebrush polymers are deposited onto mica, it becomes possible to count the number of side chains per molecule, a task that is notoriously difficult with conventional AFM techniques. Using WaveMode, our Application team achieved image quality high enough to resolve the entire molecule and accurately count the side chains. This level of detail opens new possibilities for characterizing polymer architecture at the single-molecule level.
Another example involves studying the mechanical properties of polymer particles, which requires AFM-based spectroscopy. The main challenge here is the weak adhesion of polymers to the substrate. Traditional AFM modes often push particles around during measurement, but WaveMode solves this by enabling precise control over the force applied to the sample. This makes it possible to probe mechanical properties without detaching the particles. Fast, gentle, and easy to use, WaveMode is shaping the future of polymer characterization.
